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MβCD treatment of Huh-7 cells decreases CD81 plasma membrane expression. CD81 expression was analyzed in mock-treated Huh-7 cells (A) or in Huh-7 cells after treatment with 10 mM MβCD (B). CD81 expression was also determined in MβCD-treated cells to which exogenous cholesterol was added (C). SR-BI expression was also analyzed in mock-treated (D) and MβCD-treated (E) Huh-7 cells. The bars represent 60-μm distances. These are representative data for at least two independent experiments.
