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ROCK-1 transcript abundance does not change before or after adherence. Quantitative PCR analysis was performed for 18S, ROCK-1, ROCK-2, RhoA, TNF-α and M-CSF (labeled in the graph) transcript abundance before and after adherence. Quantitative PCR analysis was performed within the linear range of amplification as determined by limited dilution and amplification of 18S ribosomal RNA. Error bars represent s.e.m. for n=3 for each transcript and condition.
