Table 2.
Primer sequences. Lower case nucleotide indicates the intentional mismatch, nucleotides in bold are located at site of SNP (where applicable), F and R indicate forward and reverse orientation.
| An. gambiae species ID | (5' to 3')Fragment (size bp) | ||
| IMP-UN: | F | GCTGCGAGTTGTAGAGATGCG | |
| R | GCATGTCCACCAACGTAAAtCC | An. quadriannulatus (637) | |
| QD-3T | R | CAACCCACTCCCTTGACGaTG | An. melas and merus (529) |
| ME-3T | R | GCTTACTGGTTTGGTCGGCAtGT | An. gambiae (464) |
| GA-3T AR-3T | R | GTGTTAAGTGTCCTTCTCCgTC | An. arabiensis (388) |
| Mopti/Savanna rDNA typing | |||
| M5 | F | CTTGGTCTGGAGACCGTTCCaTA | Mopti (426) |
| M3 | R | GACACGTCAACTAAGTCAACACATtAC | |
| S5 | F | GCCCCTTCCTCGATGGaGC | Savanna (335) |
| S3 | R | CAACCGGCCCAAACGGcTT | |
| Rdl mutation assay | |||
| RDLF | F | AGTTTGTACGTTCGATGGGTTA | positive control (256) |
| RDLR | R | CCAGCAGACTGGCAAATACC | |
| AARDL | F | GCTACACCAGCACGTGaTT | dieldrin resistant (158) |
| RDLSS | R | CAAGACAGTAGTTACACCTAAaGC | dieldrin susceptible (121) |