Table 7.
Neutralization Titers of Guinea Pigs Immunized with CON6 gp140ΔCF and gp140ΔCFI Immunogens
|
CON6
gp140ΔCF |
CON6
gp140ΔCFI |
|||||||
|---|---|---|---|---|---|---|---|---|
|
Guinea Pig Number |
Guinea Pig Number |
|||||||
| HIV-1 Isolate | 770 | 771 | 772 | 775 | 781 | 783 | 784 | 786 |
| B.BX08# | 520 | 257 | 428 | 189 | 1,930 | 390 | 2,979 | 645 |
| B.QH0692.42 | 46 | 55 | 58 | 77 | <20 | 91 | 100 | 76 |
| B.SS1196.1 | 398 | 306 | 284 | 222 | 482 | 323 | 1,151 | 446 |
| B.SF162.LS | 791 | 151 | 34 | 200 | 578 | 2,022 | 249 | 351 |
| B.JRFL-MC ** | <20 | <20 | <20 | <20 | <20 | 169 | <20 | <20 |
| B.BG1168.1 | <20 | <20 | <20 | <20 | <20 | <20 | <20 | <20 |
| B.3988.25 | <20 | <20 | <20 | <20 | <20 | <20 | <20 | <20 |
| B.6101.10 | <20 | <20 | <20 | <20 | <20 | <20 | <20 | <20 |
| CON6 | 65 | 151 | 48 | <20 | <20 | 1,308 | 41 | 260 |
50% Neutralization titers of serum after 4th or 5th immunizations as determined in the pseudotyped HIV-1 neutralization assays as described in Methods. Neutralization was considered positive (number in bold) if the titer of post-immune serum minus the titer of pre-immune bleed serum was >30. Guinea pigs sera were aslo tested as negative against 11 subtype C pseudotyped viruses including TV-1.21, DU123.6, DU172.17, 02ZM233.PB6, 92BR025.9, 02ZM197.PB7, 96ZM651.2, DU151.2, 97ZA012.29, DU422.01, DU156.12, and 2 subtype A pseudotyped viruses, 92RWO20.2 and 92UG037.02.
Assayed in Luc M7 cells using virus stock grown in PBMC.
A full-length molecular clone, and the assay stock of virus were produced by transfection in 293T cells.