Figure 3 .
Effect of IL17, TNFα, and a combination of the two cytokines on Alcian blue staining. Osteoclast-free 17 day old fetal mouse metatarsals were cultured for 11 days in the absence (A, B) or presence of IL17 (50 ng/ml) (C, D), TNFα (50 ng/ml) (E, F), or a combination of the two cytokines (G, H) and stained with Alcian blue. lhc, late hypertrophic chondrocytes; ehc, early hypertrophic chondrocytes; pc, proliferating chondrocytes; rc, resting chondrocytes. Magnification: A, C, E, G x40; B, D, F, H x200. Cultures were performed in quadruplicate (n=4).