Figure 2 .
O-glycosidase. HeLa protein western blot. Lanes 1 and 2 preincubated with water, 3+4 with N-glycosidase F, 5-6 with O-glycosidase. Subsequent incubation with anti-p68-positive RA serum (2, 4, 6) or anti-p68-positive rabbit serum. Detection with FITC conjugated secondary antibody. p68 was detectable by the RA serum antibody after incubation with water and with N-glycosidase F and not after incubation with O-glycosidase (5). p68 was detectable by the rabbit serum antibody even after treatment with O-glycosidase (6).