Figure 2.
Retroviral expression of LXRα in RAW264.7 cells facilitates the induction of ABCA1 and ABCG1 mRNA in response to LXR and RXR ligands. (A) RAW264.7 cells were transduced with retroviral vector alone or LXRα expression vector as described in Materials and Methods. Cells were incubated for 24 h in medium A containing the indicated concentration of 20(S)-OHC and/or LG268 (50 nM). Northern analysis was performed as in Fig. 1. A cDNA probe for GAPDH was used as a control to ensure equivalent loading and integrity of RNA (not shown). (B) Quantitation of ABCA1 and ABCG1 mRNA levels shown in part A normalized for GAPDH expression. mRNA levels in untreated RAW-vector cells were arbitrarily assigned value of 1.0.