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Variation in F-luc activity of K5290 mutants. The graph shows the F-luc expression of the indicated constructs expressed as a fraction of the expression of wild-type F-luc. The mutants include a deletion of the entire firefly luc gene (Fluc) and those replacing the K529 codon (AAA) with the indicated codon. Indicated below each codon is the amino acid it encodes. Error bars are standard errors of the mean.
