TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli strains | ||
| i484 | O25:H autoagluttinating; human isolate | 18 |
| xy006 | i484, fitA::EZ-TN5 <Kan-2> | This study |
| xy005 | i484, fitB::EZ-TN5 <Kan-2> | This study |
| xy009 | i484, fepA::EZ-TN5 <DHFR> | This study |
| xy008 | i484, fepC::EZ-TN5 <DHFR> | This study |
| xy007 | i484, fhuA::EZ-TN5 <DHFR> | This study |
| oy016 | i484, lacZ::EZ-TN5 <DHFR> | This study |
| AA93 | F−araD139 ΔlacU169 rpsL150 relA1 deoC1 flbB5301 ptsF25 rbsR aroB fecB::Mud1 (Ap lac) | 26 |
| oy097 | AA93; feoB::EZ-TN5 <DHFR> | This study |
| AB1515.199 | purE42 proC14 leu-6 trpE38 thi-1 fhuA23 lacY1 (fepC::Tn5) | 6 |
| 123 | Field isolate | Pig |
| 124 | Field isolate | Pig |
| 252 | Field isolate | Pig |
| EcoR33 | Field isolate | Pig |
| 263 | Field isolate | Pig |
| 431 | Field isolate | Pig |
| 987 | Field isolate | Pig |
| 1413 | Field isolate | Pig |
| 16 | Field isolate | Human |
| 17 | Field isolate | Human |
| 18 | Field isolate | Human |
| EcoR40 | Field isolate | Human |
| EcoR60 | Field isolate | Human |
| EcoR62 | Field isolate | Human |
| EcoR8 | Field isolate | Human |
| EcoR26 | Field isolate | Human |
| EcoR42 | Field isolate | Human |
| EcoR45 | Field isolate | Human |
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdS17 (rK− mK+) deoR thi-1 supE44 λ−gyrA96 relA1 | Invitrogen |
| Plasmids | ||
| pMP220 | Contains a promoterless lacZ gene, Tetr IncP | 33 |
| pMP-fitA | fit promoter cloned into pMP220 with direction of fitA transcription | This study |
| pMP-fitB | fit promoter cloned into pMP220 with direction of fitB transcription | This study |
| pCR | Cloning plasmid pCR-XL-TOPO | Invitrogen |
| pCR63 | lacZ cloned into pCR-XL-TOPO | This study |
| pCR64 | fitR cloned into pCR-XL-TOPO | This study |
| pfit1 | Whole fit region cloned into pCR-XL-TOPO | This study |