Fig. 4.
Transcription of 65% linear chromatin requires presence of all critical components of the system. Negatively supercoiled (−SC) or linear (Lin.) pLY10 DNA, or 65% chromatin assembled on linear pLY10 (Lin. Chr.) were transcribed as described in the Fig. 1A. The reactions contained all components required for enhancer-dependent transcription, or one of the components was missing as indicated. Note that in all cases the specific transcripts are formed only when the reactions contain all critical components of the system. M, end-labeled pBR322-MspI digest.