Figure 5.
Removal of the NK2-SD activates Nkx2.2. A reporter plasmid containing seven tandem copies of the Nkx2.2 DNA-binding consensus site (shown in Table 1) upstream of the prolactin promoter driving luciferase (pFOxLuc1–7XNk2) was cotransfected with the CMV promoter-driven expression plasmids encoding the Nkx2.2 cDNA shown. Relative luciferase activities are calculated, with the activity of cells transfected with a CMV expression plasmid without cDNA insert set at 1. All data are shown as mean ± SEM.