Figure 3.
lpg1− cells express normal levels of PPG. (A) PPG was purified from culture supernatants or parasites fractionated by Triton X-114 extraction and subjected to Western blot analysis with anti-PG antibody WIC 79.3. (B) Indirect immunofluorescence microscopy is shown with anti-PG antibody WIC79.3. The exposure time for WT was 1/15 s, whereas for lpg1− it was 1/4 s. The bar corresponds to 5 μm. (C) Flow cytometry of fixed permeablized parasites labeled with anti-phosphoglycan antibody WIC 79.3. Control represents cells incubated only with secondary antibody.