Figure 2.
Domains and PCTD binding of CA150. (A) Upper open box represents the 1,098-aa CA150 protein, with WW domains shaded black and FF motifs, numbered sequentially, shaded gray. Gray boxes in second row labeled WW and A represent segments of protein expressed in bacteria and tested for PCTD binding in B. Construct names and amino acid numbers: WW (); A (631–1098). Relative binding affinities to the PCTD, as judged by eye from Far Western analyses, are indicated below each construct. (B) GST fusion proteins carrying segments WW and A were run in two different amounts (1× = ≈100 ng for WW and ≈50 ng for A) on duplicate SDS gels that were either stained (Coomassie) or blotted to nitrocellulose and probed with 32P-labeled PCTD (Far Western). Prestained marker positions are indicated at left (apparent Mr). The amino terminal-most segment, containing WW1, was not successfully expressed in bacteria.