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. 2003 Aug;77(16):8729–8735. doi: 10.1128/JVI.77.16.8729-8735.2003

TABLE 1.

Cytokine secretion profiles of antigen-stimulated splenocytesalegend

Stimulation Level of cytokine secretion (pg/ml)
IFN-γ IL-2 IL-4 IL-10 TNF-α
Mock 2,800 ± 932 1,887 ± 381 66 ± 17 263 ± 9 29 ± 0
Sham 4,399 ± 729 2,487 ± 450 109 ± 9 284 ± 10 34 ± 2
gp120 172,144 ± 50,77 32,793 ± 6,459 515 ± 72 570 ± 81 34 ± 3
gp120/GM 183,127 ± 41,181 38,983 ± 2,396 503 ± 72 771 ± 21 37 ± 1
gp120/MIP 286,344 ± 62,971 45,862 ± 4,800 513 ± 43 610 ± 49 37 ± 3
gp120/GM/MIP 1,858,355 ± 500,472 199,279 ± 43,340 306 ± 66 813 ± 91 39 ± 1
legend

aGroups of mice (n = 4 per group) were primed with a sham plasmid, the gp120 DNA vaccine alone, or cytokine-augmented DNA vaccines. These mice were then boosted at week 8 with DNA and at week 24 with 106 particles of rAd5. Levels of cytokine secretion (picograms per milliliter) by recombinant gp120-stimulated splenocytes were assessed by ELISA. Splenocytes from naïve mice (mock) were included as a control to determine baseline levels of cytokine secretion.