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Genetic complementation of the dspB mutation in A. actinomycetemcomitans strain JK1023: colony morphology on agar (A and B) and biofilm dispersal phenotype in broth (C and D) of strain JK1023 harboring vector plasmid pJAK16 (A and C) or the complementary plasmid pJK618 (B and D). Bar = 1 mm. The cells in panels C and D were grown in the wells of six-well microtiter plates (diameter, 35 mm; Falcon no. 353046) for 5 days and stained with crystal violet as previously described (16).
