FIG. 5.
Accumulation of GFP-XPA within restricted nuclear areas after local UV irradiation. (A) Schematic presentation of local UV damage infliction on living cultured cells. (B and C) Micrographs (phase-contrast image [B] and fluorescence image [C]) of living cells expressing GFP-XPA (clone 40) and UV irradiated through a filter with small (5-μm-diameter) pores. The arrows in panel C point to the local accumulations of GFP-XPA. (D and E) GFP-XPA accumulations (arrows) shown in panel D clearly colocalize with endogenous XPC (E) concentrations, as determined with anti-XPC antibodies, in fixed cells.