FIG. 1.
Functionality of GFP-XPA. (A) Schematic representation of the His9-HA-eGFP-XPA fusion gene with the different binding domains indicated. NLS, nuclear localization signal; BD, binding domain; aa, amino acids. (B) Fluorescence image of XP-A cells injected with GFP-tagged XPA cDNA. Only the multinucleated cell microinjected with GFP-XPA cDNA showed a homogeneous nuclear expression (number 1); surrounding cells were not injected (number 2). (C) Measurement of the repair capacity of cells with fluorescent nuclei by means of UV-induced UDS (see Materials and Methods). The amount of silver grains above the nuclei of the injected cells (number 1) was comparable to what was seen with wild-type cells (not shown), whereas the surrounding XP-A fibroblasts (number 2) show the low level of DNA synthesis typical for UV-exposed XP-A cells. The cell indicated with the number 3 is in S phase.