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Lack of complementation of temperature-sensitive growth and MMS sensitivity of strain rad27Δ. Exponentially growing yeast cultures of W303 wild type, rad27Δ, rad27Δ/vector, rad27Δ/pGAL-hBLM, and rad27Δ/Yep24-Rad27 were spotted in 10-fold serial dilution onto plates. (A) Growth at 30°Cor37°C. (B) Growth in the presence and absence of 0.005% MMS. Cells were grown for 3 days.
