TABLE 1.
Melting temperatures for Mycobacteriumstrains in this study
| Species | Strain(s)a (n) | Mean melting temp (°C) of oligonucleotide specific forb:
|
||
|---|---|---|---|---|
| M. tuberculosis narGHJI (SD) | Bovine mycobacteria oxyR (SD) | M. bovis BCG RD1 (SD) | ||
| M. tuberculosis | H37Rv ATCC 25618 | 56.8 | 68.0 | 62.6 |
| Erdmann ATCC 35801 | 57.0 | 68.3 | 62.1 | |
| Clinical strains (33) | 56.9(0.36) | 68.2 (0.15) | 61.9 (0.47) | |
| M. africanum | Clinical strains (3) | 63.4 (0.21) | 68.0 (0.28) | 62.4 (0.49) |
| M. microti | Clinical strains (2) | 63.2 (0.28) | 68.2 (0.07) | 61.7 (0.07) |
| M. bovis | ATCC 19210 | 63.1 | 59.3 | 62.5 |
| Clinical strains (12) | 63.2 (0.30) | 59.2 (0.13) | 61.7 (0.26) | |
| M. bovisBCG | Pasteur ATCC 35734 | 63.6 | 59.7 | 49.4 |
| Copenhagen ATCC 27290 | 63.5 | 59.0 | 49.6 | |
| Moreau ATCC 35736 | 63.7 | 59.2 | 49.5 | |
| Tice ATCC 35743 | 63.5 | 59.0 | 49.5 | |
| Connaught ATCC 35745 | 63.7 | 59.1 | 49.4 | |
| Clinical strains (4) | 63.2 (0.26) | 59.2(0.09) | 49.6(0.14) | |
a
Strains were obtained from the ATCC or various sources as indicated in Materials and Methods. Bacilli were cultured in the MGIT automated culture system, and DNA was prepared by mechanical disruption and used directly for LightCycler amplification.
b
For ATCC strains, individual melting temperatures are shown. For all clinical strains, the standard deviations are given in parentheses for each group of strains. Melting temperatures specific for each Mycobacterium group are indicated in boldface in the respective columns.