FIG. 4.
LightCycler analysis of the polymorphic nucleotide at position −215 within the promoter region of narGHJI of M. tuberculosis, M. bovis, M. bovis BCG, M. africanum, and M. microti. Strains were cultured in the MGIT automated culture system, and DNA was prepared from an aliquot of positive culture by mechanical disruption. Melting peak analysis of the amplification product was done after the last amplification cycle. The melting curve analysis is displayed as the first negative derivative of the fluorescence (−dF/dT) versus temperature. F2 refers to channel 2, which is used by the LightCycler's optical unit to measure signals from LightCycler Red 640 at 640 nm.