TABLE 1.
Primers for quantitation of inflammatory genes
| Primer | Sequence (5′ to 3′) | Accession no. | Concn (nM) | Calibrator gene |
|---|---|---|---|---|
| IL 8-F | GACCACACTGCGCCAACA | AF385628.2 | 15 | GAPDH |
| IL 8-R | GCTCTCTTCCATCAGAAAGTTACATAATTT | 15 | ||
| GROα-F | GCCCAAACCGAAGTCATAGC | NM_001511.1 | 2.5 | RPII |
| GROα-R | GAGGCAAGCTTTCCGCC | 45 | ||
| GROγ-F | CCGGACCCCACTGCG | M36821 | 2.5 | G6PD |
| GROγ-R | TTCCCATTCTTGAGTGTGGCTA | 15 | ||
| IP10-F | AGTGGCATTCAAGGAGTACCTCTC | NM_001565.1 | 15 | RPII |
| IP10-R | GATGCAGGTACAGCGTACGGT | 2.5 | ||
| MIP1α-F | CTACTTTGAGACGAGCAGCCAG | NM_002983.1 | 45 | G6PD |
| MIP1α-R | GGTTAGGAAGATGACACCGGG | 15 | ||
| RANTES-F | CTACACCAGTGGCAAGTGCTC | NM_002985.2 | 15 | G6PD |
| RANTES-R | TTTCGGGTGACAAAGACGACT | 2.5 | ||
| TNFα-F | GGACCTCTCTCTAATCAGCCCTC | NM_000594 | 2.5 | GAPDH |
| TNFα-R | TCGAGAAGATGATCTGACTGCC | 2.5 | ||
| IL-6-F | CGGTACATCCTCGACGGC | NM_000600 | 2.5 | GAPDH |
| IL-6-R | CTTGTTACATGTCTCCTTTCTCAGG | 45 | ||
| IL1β-F | CTCCACCTCCAGGGACAGGA | BT007213.1 | 45 | EEF1γ |
| IL1β-R | GGACATGGAGAACACCACTTGTT | 15 | ||
| IL-10-F | GAGGCTACGGCGCTGTCA | AY029171.1 | 45 | GAPDH |
| IL-10-R | GCCTTGCTCTTGTTTTCACAGG | 15 | ||
| GAPDH-F | GTGGAGTCCACTGGCGTCTT | J04038 | 2.5 | |
| GAPDH-R | GCAAATGAGCCCAGCCTTC | 15 | ||
| EEF1γ-F | AAACTGTGTGAGAAGATGGCCC | NM_001404.3 | 15 | |
| EEF1γ-R | GGGTCTCTGCAAACTTTTTAGCA | 2.5 | ||
| G6PD-F | GCCAAGAAGAAGATCTACCCCA | NM_000402.2 | 15 | |
| G6PD-R | AAGGCCATCCCGGAACAG | 2.5 | ||
| RPII-F | TCGAGCAGATCAGCAAGGTG | NM_000937.2 | 2.5 | |
| RPII-R | TCTTGTTGTCTGTCTGTGGCAA | 2.5 |