Figure 3.
Induction of FRQ alone is sufficient to phase shift the clock and to trigger singularity behavior. (A) Race tube assays showing the phase shift of the circadian conidiation rhythm after a 3 h treatment of QA at DD28. A wild-type strain containing a qaFRQ construct was used in the experiments. (B) Race tube assays showing the singularity behavior after the QA treatment at DD26. At indicated time points, QA was added to the cultures for 2 h before the cultures were washed and inoculated onto race tubes. (C) Race tube assays showing that the QA treatment at DD26 did not phase shift the rhythm for a wild-type strain without the qaFRQ construct. (D) PRC of a 2 h QA treatment for the wt,qaFRQ strain. The phase shifts of individual race tubes for DD26 and DD27 are shown.