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. 2006 Oct 30;103(45):16687–16691. doi: 10.1073/pnas.0607911103

Fig. 2.

Fig. 2.

Hydrolysis of O-acetyl-ADP-ribose by ARH3. (A) Hydrolysis of O-acetyl-ADP-ribose by ARH3. Assays containing 1.5 pmol of mouse ARH3 and 2.5 μM O-acetyl-[14C]ADP-ribose, 50 mM potassium phosphate (pH 7.0), 10 mM MgCl2, and 5 mM DTT (total volume 200 μl) were incubated at 30°C for the indicated time before separation of substrate and products by using RP-HPLC as described in Materials and Methods. Data are means ± 1/2 the range of values from duplicate assays. Results were similar in two experiments. (B) Assays containing 2.5 μM O-acetyl-[14C]ADP-ribose and the indicated amount of mouse ARH3 in 200 μl of buffer were incubated for 1 h at 30°C before separation of substrate and products by using RP-HPLC as described in Materials and Methods. Data are means ± 1/2 the range of values from duplicate assays. Results were similar in two experiments.