TABLE 2.
Summary of rifampin-induced transcription modulationa
| Strain description | Modulation in indicated reporter
|
|||||||
|---|---|---|---|---|---|---|---|---|
| fliA | flhB | cirA | STM1328 | STM3595 | spvAB | ucpA | talA | |
| 14028 | ↓↓ | ↓↓ | ↓↓ | ↓ | ||||
| 14028 crp::Tn10 | ↓ | *b | ↓↓ | ↓ | **c | |||
| 14028 fis::tet | ↓ | * | ↓ | ↓ | ↓** | ↓↓** | ** | |
| 14028 hns::Tn10 | ↓↓↓ | ↓↓ | ↓ | ↓ | ||||
| 14028 ihfB::cat | ↓↓↓ | ↓↓ | ↓ | ↓ | * | |||
| 14028 fnr::Tn10 | ↓↓ | ↓↓ | ↓↓ | ↓↓↓ | ↑ | |||
| 14028 rpoS::amp | ↓↓ | ↓ | ↓↓ | ↓↓↓ | * | |||
The modulation is depicted by up arrows (RIUR) and down arrows (RIDR) as follows: a 2.5- to 5-fold effect is depicted as one arrow, a 6- to 15-fold effect by two arrows, and a 16-fold or greater effect as three arrows. No arrow indicates there was a less-than-2.5-fold difference.
In four cases, indicated by single asterisks, the introduction of the mutations changed RITM from strong RIDR to no effect.
In four cases, indicated by double asterisks, the mutation of a global regulator either blocked RIUR or switched the response from RIUR to RIDR, indicating that the regulator was involved in RIUR for that promoter.