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Kinetics of Cr(VI) reduction for wild-type E. coli ChrR and the evolved ChrR6 enzyme
| Enzyme | Vmax [nmol Cr(VI) mg protein−1 min−1] | Km (μM) | kcat (s−1)a | kcat/Km |
|---|---|---|---|---|
| E. coli ChrR | 295 ± 27 | 376 ± 14 | 30 ± 2 | 4.5 × 104 ± 3 × 103 |
| ChrR6 | 8,812 ± 611 | 41 ± 5 | 521 ± 18 | 1.3 × 107 ± 3 × 105 |
Based on a dimeric enzyme molecular mass of 50 kDa (1).
