Figure 3.
The cytoplasmic tail of β4 integrin is essential for β4 integrin–mediated keratinocyte directional migration in the absence of EGF. β4+, β4+CTs were starved for 16 h in basal medium containing no growth factors, and experiments were performed as described in the legend for Figure 1. The cosine of migration for β4+ and β4+CT cells in the presence and absence of EGF is shown. The number of cells is as follows: β4−, no EGF, n = 64; β4+, no EGF, n = 113; β4+CT, no EGF, n = 116; β4−, EGF, n = 98; β4+, EGF, n = 81; and β4+CT, EGF, n = 141. The data shown are combined from at least four independent experiments. Error bars, SEM. *p < 0.01 between all conditions and β4+ in the presence of EGF. #p < 0.01 between β4+ in the absence of EGF and both β4− and β4+CT in the absence of EGF.