Figure 2.
Laminin 332–mediated ligation of β4 integrin is required for keratinocyte directional migration in the absence of EGF. β4+, β4+ADs were starved for 16 h in basal medium containing no growth factors, and experiments were performed as described in the legend for Figure 1. (A) The cosine of migration for β4+ and β4+AD cells in the presence and absence of EGF is shown. The number of cells is as follows: β4−, no EGF, n = 64; β4+, no EGF, n = 113; β4+AD, no EGF, n = 73; β4−, EGF, n = 98; β4+, EGF n = 81; and β4+AD EGF, n = 82. The data shown are combined from at least four independent experiments. Error bars, SEM. *p < 0.01 between all conditions and β4+ cells in the presence of EGF. (B) β4+ and NHKs were starved for 16 h in basal medium containing no growth factors, and experiments were performed as described in the legend for Figure 1. The cosine of migration for β4+ cells in the presence and absence of ASC-8 and EGF is shown. The number of cells is as follows: β4−, no EGF, n = 64; β4+, no EGF, n = 113; β4+, no EGF + ASC-8, n = 75; β4−, EGF, n = 98; β4+, EGF, n = 81; and β4+, EGF +l ASC-8, n = 114). The data shown are combined from at least four independent experiments. Error bars, SEM. *p < 0.01 between all conditions and β4+ in the presence of EGF. (C) The cosine of migration for NHKs in the presence and absence of ASC-8 and EGF is shown. The number of cells is as follows: NHK, no EGF, n = 59; NHK, no EGF + ASC-8, n = 70; NHK, EGF, n = 78; and NHK, EGF + ASC-8, n = 62. The data shown are combined from at least four independent experiments on three separate cell strains. Error bars, SEM. *p < 0.01 between all conditions and NHK in the presence of EGF.