Figure 2.
Immunocytochemical staining of endogenous calmodulin in isolated pancreatic acinar cell. (Bar = 20 μm.) (A) Two unstimulated cells stained for calmodulin by using antibody clone 6D4 from Sigma, followed by a secondary antibody labeled with a Cy3 fluorescent marker. In both cells, calmodulin labeling can be seen throughout the cytosol and SG region. (B) An example of two cells that have been stimulated with 1 nM CCK for 150 s and then incubated in anticalmodulin antibody; the nuclei in both cells are labeled strongly for calmodulin.