FIG. 5.
GATA-1-regulated Cdk protein associations. G1E-ER4 whole-cell lysates were immunoprecipitated using antibodies indicated at the bottom of each panel and analyzed by Western blotting. (A) Cdk4 IP. Positive control (pos ctl): 20% input protein; 0-h samples for Cdk4, cyclins D2 and D3; 24-h samples for p18, p19, and p27. Negative control (neg ctl): IP using isotype-specific antibody (anti-rabbit IgG) against 0 h samples for Cdk4, cyclins D2 and D3 and 24-h samples for p18, p19, and p27. (B) Cdk6 IP. Controls were the same as for panel A, except that pos ctl represents 10% input. (C) p18 IP. Controls were the same as for panel A, except that pos ctl represents 5% input. (D) Cdk2 IP. Controls were the same as for panel B.