Figure 6.
POMC promoter function in repressed (Dex) and activated (CRH) conditions. (A) Initiation of POMC transcription as measured in nuclear run-on assays in pituitary primary culture treated with Dex and/or CRH. (B) ChIP analyses of RNA Pol II recruitment to the POMC promoter and exon 3 in control and treated AtT-20 cells. (C) ChIP analysis of POMC gene occupancy by Pol II with phospho-Ser2 CTD. (D) ChIP analysis of POMC gene occupancy by Pol II with phospho-Ser5 CTD. (E) ChIP analysis of acetylated histone H4 over the POMC promoter and exon 3. Dashed lines indicate background ChIP signals.