Figure 3.
Tumor heterogeneity. A: CD10+/CD13+ tumor glands. Two specimens, 01–089D and 01–090D, are contrasted. In the top panels, the cancer glands (middle) of 01–089D are not stained for CD10 and CD13 (whereas surrounding benign glands are stained). In the bottom panels, some of the small cancer glands in 01–090D are stained for CD10, and all for CD13. The cancer glands are negative for CD104 (not shown). Magnification is ×100. B: Adjacent, differentially stained tumor cell populations of high Gleason grade. Serial sections of specimen 01–081D were stained with the antibodies identified in the individual micrographs. Two tumor CD phenotypes are evident. Both types are positive for CD75s and negative for CD104 (stained are basal cells of benign glands, endothelial cells of blood vessels, and nerve cells in this field). The two types are otherwise indistinguishable by histomorphology. The Gleason score given for this specimen is 4 + 4. Magnification is ×100. C: Adjacent differentially stained tumor glands of low Gleason grade. One type of tumor glands is scored as CD10−/CD13−/CD57− (blue arrow) while the other is scored as CD10+/CD13+/CD57+ (red arrow) in specimen 01–076D. Both cancer gland types are CD24+/CD26+/CD38+/CD75s+/CD104−. The Gleason score given for this specimen is 3 + 3. Magnification is ×100.