Figure 4.
The presence of fascin (I), β-tubulin (II), HSC70 (III), and HSP90 (IV) were confirmed by Western blots of PMR-MM7-derived exosomes (A) and PMR-MM8-derived exosomes (B). The primary antibodies anti-fascin (1:1000), anti-β-tubulin (1:10.000), and anti-HSP90 (1:2000) were followed by horseradish peroxidase-conjugated goat anti-mouse IgG1. Anti-HSC70 (1:1000) was followed by Envision (DAKO). Blots were incubated with SuperSignal West Pico chemiluminescent substrate and exposed to Hyperfilm ECL (Amersham Biosciences, Buckinghamshire, England).