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. Author manuscript; available in PMC: 2006 Oct 16.
Published in final edited form as: J Biol Chem. 2006 Jun 9;281(31):21884–21891. doi: 10.1074/jbc.M600427200

Figure 6.

Figure 6

Binding of thioredoxin-GST beads to Txnip N-terminal Cys-to-Ser mutants. Wildtype and mutant Txnip proteins were overexpressed in 293 cells and the cell lysates were incubated with thioredoxin-C73S-GST. After washing, beads were incubated in sample buffer and the supernatant was probed for Txnip with JY2. Input cell lysates were probed for Txnip and demonstrated similar immunoreactivity. Similar amounts of thioredoxin-GST were observed in the supernatant by Coomassie staining. The experiment was replicated with essentially identical results as those shown.