Figure 6.
Increased E-cadherin level and its continued interaction with β-catenin is observed in pancreatic adenocarcinoma. (A) Two pairs of tumors and normal pancreata atthe upper left panel show increased levels of total E-cadherin in this representative Western blot analysis. The right pair shows no change. The bottom panels show β-actin as a loading control. (B) A representative coprecipitation study shows continued β-catenin-E-cadherin association (middle panel) in pancreatic adenocarcinomas in most tumors, which was a function of immunoprecipitated β-catenin in lysates (bottom panel). No tyrosine phosphorylation of β-catenin was detectable (top panel).