FIG. 2.
BFT metalloprotease activity is necessary for binding to HT29/C1 cells. Preincubation of Alexa 488-labeled BFT (6 to 10 nM) in cell culture medium with the general metalloprotease inhibitor GM6001 (5 μM) for 30 min prior to addition to HT29/C1 cells significantly inhibits BFT binding compared to Alexa 488-labeled BFT alone (6 to 10 nM, 1 h, 37°C) (P < 0.001). In contrast, incubation of HT29/C1 cells for 30 min with GM6001 (5 μM) with washing prior to incubation with Alexa 488-labeled BFT as described above does not inhibit BFT binding compared to Alexa 488-labeled BFT alone (P was not significant [NS]). Four experiments were performed.