TABLE 2.
Reproducibility of the chipa
| Codon | Expected amino acid | Amino acid determined by the chip in experimentb:
|
Mean It/b ratio | SD | CV (%) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | |||||
| rt173 | V | V | V | V | V | V | V | V | V | V | V | V | 45.81 | 7.43 | 16.2 |
| rt180 | L | L | L | L | L | L | L | L | L | L | L | L | 75.7 | 14.44 | 19.1 |
| rt204 | M | ind | ind | ind | ind | ind | ind | ind | ind | ind | ind | ind | 1.44 | 0.31 | 21.9 |
| rt207 | V | V | V | V | V | V | V | V | V | V | V | V | 51.87 | 7.48 | 14.4 |
| rt120 | P | P | P | P | P | P | P | P | P | P | P | P | 10.61 | 2.51 | 23.7 |
| rt129 | Q | ind | ind | ind | ind | ind | ind | ind | ind | ind | ind | ind | 1.05 | 0.17 | 16.2 |
| rt145 | G | G | G | G | G | G | G | G | G | G | G | G | 22.57 | 4.45 | 19.7 |
| rt171 | S | S | S | S | S | S | S | S | S | S | S | S | 75.70 | 14.44 | 19.1 |
| Precore 28 | W | W | W | W | W | W | W | W | W | W | W | W | 18.05 | 2.90 | 16.1 |
| Precore 29 | G | G | G | G | G | G | G | G | G | G | G | G | 16.50 | 3.26 | 19.8 |
Genotyping results showed similar reproducibility: The genotype determined by the chip in all 11 experiments was D, which was the expected genotype. For the genotyping experiments, the mean It/b ratio was 72.90% (standard deviation [SD], 1.13%) and the CV was 1.55%.
ind, indeterminate result.