Figure 3.
Melan-a transformation is accompanied by an increase in tri-antennary and tetra-antennary oligosaccharides and by modification in β1 integrin chain. (A) Cell surface tri-antennary and tetra-antennary oligosaccharide expression on melan-a (ma), 2C, 4C, 4C3, 4C11, Tm1, and Tm5 cell surfaces analyzed by flow cytometry using biotin-L-PHA lectin and FITC-streptavidin. (B) Proportion of L-PHA-positive cells (same experiment as in A) (C). Glycoproteins from cell extracts containing tri-antennary and tetra-antennary N-glycans, as shown by lectin blotting using L-PHA lectin. Analysis by flow cytometry of tri-antennary and tetra-antennary oligosaccharide expression, as recognized by L-PHA lectin (D). β1 integrin expression on melan-a (ma), Tm5, and B16F10 cells visualized by Western blot analysis, using a specific polyclonal antibody.