FIGURE 2. siRNA directed against p85α or p110α inhibits proliferation. The effect of siRNA directed to PI3K components on the viability of KM20 (A) or HT29 (B) cells was assessed. Cell viability was measured as described in Materials and Methods. Points represent means of triplicate determinations ± SD. *P < 0.05 for p85α, p110α siRNA compared with nontargeting control (NTC) siRNA. KM20 (C) or HT29 (D) cells transfected with p85α, p110α, or NTC. siRNA sequences were lysed and Western blots performed using anti-Akt, phospho (Ser473), anti-p85α, and anti-p110α; β-actin was used as a loading control (bottom row).