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Effect on the IR-induced G2/M checkpoint of the bre1Δ, dot1Δ, and rad9Δ mutations compared to wild type. Cells were released from nocodazole synchronization into fresh medium at time zero after 500 Gy of 137Cs gamma irradiation, and in parallel without irradiation. The percentage of cells that have undergone nuclear division is shown for each culture at 30-min intervals thereafter. Strains used were g1295-21C bre1Δ, g1295-6C dot1Δ, g1304-19C rad9Δ, and BY4741 wild-type MATa.
