FIG. 2.
Detection of endogenous PML by Western blot analysis of cell lysates derived from primary human fibroblasts transduced with various siRNA expression vectors as indicated. (A) Upper panel: monoclonal anti-PML antibody 5E10 was used to detect PML; lower panel: beta-actin was detected as a loading control. (B) Comparison of the reactivities of anti-PML monoclonal antibody 5E10 and anti-PML rabbit serum H-238 using cell lysates from primary human fibroblasts transduced with pSIREN-RetroQ vectors as indicated. Lanes 1 to 3, upper panel: monoclonal 5E10; lower panel: beta-actin antibody. Lanes 4 to 6, upper panel: anti-PML rabbit serum H-238; lower panel: anti-Daxx rabbit serum M-117. The localization of PML isoforms is indicated on the right of each panel; the asterisks indicate a nonspecific reaction of anti-PML antibody 5E10. Numbers at left of each panel are molecular masses in kilodaltons.