TABLE 2.
Recombinant NDV strains are highly lytic to tumor cells in vitroc
| Cell line | rLaSotaa
|
rLaSota V.F.a
|
rBCa
|
rBC-EGFPa
|
rBC-Edita
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Sensitivityb (%) | MOI | Sensitivity (%) | MOI | Sensitivity (%) | MOI | Sensitivity (%) | MOI | Sensitivity (%) | MOI | |
| THP-1 | 0 | >10 | 100 | 0.0001 | 100 | 0.003 | 100 | 0.001 | 100 | 0.04 |
| CCRF-CEM | 0 | >10 | 100 | 0.283 | 0 | >10 | 0 | >10 | 0 | >10 |
| Leukemia cell lines | 0 | >10 | 100 (2/2) | 0.142 | 50 (1/2) | 0.003 | 50 (1/2) | 0.001 | 50 (1/2) | 0.04 |
| Prostate cancer (PC3) | 0 | >10 | 100 (1/1) | 0.0005 | 100 (1/1) | 0.0001 | 100 (1/1) | 0.01 | 100 (1/1) | 0.0019 |
| HT1080 | 0 | >10 | 100 (1/1) | 3.5562 | 100 (1/1) | 0.02 | 100 (1/1) | 0.01 | 0 | >10 |
| HuTu80 | 0 | >10 | 100 (1/1) | 0.21725 | 100 (1/1) | 0.007 | 100 (1/1) | 0.01 | 100 (1/1) | 0.019 |
| Cervical cancer (HeLa) | 100 | 10 | 100 (1/1) | 0.01 | 100 (1/1) | 0.72 | 100 (1/1) | 1.0 | 100 (1/1) | 0.007 |
| Liver cancer (HEpG2) | 100 | 1.0 | 100 (1/1) | 0.0164 | 100 (1/1) | 0.019 | 100 (1/1) | 0.1 | 100 (1/1) | 0.0022 |
| Caco-2 | 0 | >10 | 100 | 0.0573 | 0 | >10 | 0 | >10 | 100 | 0.00004 |
| CoLo 205 | 0 | >10 | 100 | 0.0076 | 0 | >10 | 0 | >10 | 0 | >10 |
| SW 620 | 0 | >10 | 100 | 3.03 | 100 | 0.988 | 100 (1/1) | 1.0 | 100 | 1.081 |
| HT29 | 0 | >10 | 100 | 4.648 | 0 | >10 | 0 | >10 | 100 | 0.0006 |
| T84 | 0 | >10 | 0 | >10 | 0 | >10 | 0 | >10 | 100 | 0.0391 |
| Colon cancer cell lines | 0 | >10 | 80 (4/5) | 1.9357 | 20 (1/5) | 0.988 | 20 (1/5) | 1.0 | 80 (4/5) | 0.2802 |
| SH-SY5Y neuroblastoma | 0 | >10 | 100 | 0.002 | 100 | 0.0025 | 100 (1/1) | 0.01 | 100 | 0.0004 |
| Breast carcinoma (MCF-7) | 0 | >10 | 0 (0/1) | >10 | 0 (0/1) | >10 | 0 (0/1) | >10 | 0 (0/1) | >10 |
| All cell lines tested | 14 (2/14) | >10 | 86 (12/14) | 0.84 | 57 (8/14) | 0.22 | 57 (8/14) | 0.27 | 71 (10/14) | 0.12 |
a
In parentheses are numbers of highly susceptible cell lines out of the number of cell lines tested. A cell line was considered highly susceptible if the EC50 was ≤MOI of 1 following a 48-h infection. The MOI represents the average EC50 (MOI) of susceptible cell lines obtained from two independent experiments. All of the tested viruses are recombinant viruses derived by our established reverse genetics techniques.
b
The sensitivity is the percentage of cancer cell lines by tumor type deemed highly sensitive to virus-mediated killing.
c
Boldface type indicates combined results for indicated cell lines.