Figure 9. S1P-evoked ML-1 cell migration is regulated by Rho and ROCK.
Experiments were performed in the absence (A and B) and presence (C and D) of 10% (v/v) FCS. (A and C) Cell migration in control cells (white bars) or cells preincubated with C3 exotoxin (100 ng/ml, 4 h, hatched bars). (B and D) Cell migration in control cells (white bars) or cells preincubated with Y-27632 (10 μM, 1 h; black bars). * indicates statistically significant differences as indicated by parentheses. ◆ indicates statistically significant differences compared with control cells treated with Y-27632 or C3 exotoxin. Results are means±S.E.M., n=3. *P<0.05; **P<0.01; ***/◆◆◆P<0.001 (one-way ANOVA and Holm's post hoc test).