Table 2.
ssDNA-binding parameters of PriB
| Apparent Kd (nM) | h | |
|---|---|---|
| dT10 | 740 ± 70 | 1.2 ± 0.1 |
| dT15 | 100 ± 20 | 1.5 ± 0.1 |
| dT20 | 30 ± 10 | 1.6 ± 0.1 |
| dA10 | 1280 ± 100 | 1.2 ± 0.1 |
| dA15 | 490 ± 40 | 1.3 ± 0.1 |
| dA20 | 290 ± 30 | 1.6 ± 0.1 |
| dA25 | 210 ± 20 | 1.4 ± 0.2 |
| dA30 | 120 ± 20 | 2.6 ± 0.2 |
| dA35 | 110 ± 20 | 3.0 ± 0.6 |
| dA40 | 70 ± 20 | 2.8 ± 0.5 |
| dA45 | 70 ± 20 | 2.7 ± 0.4 |
| dA50 | 40 ± 10 | 2.6 ± 0.4 |
| dA55 | 40 ± 10 | 2.8 ± 0.1 |
| dA60 | 40 ± 10 | 2.8 ± 0.2 |
| dA65 | 40 ± 10 | 2.9 ± 0.3 |
The errors are standard deviations determined using 2–4 independent titration experiments.