Fig. 1.
Generation of adiponectin-deficient mice. A: gene-targeting strategy. Homologous recombination of the targeting vector with the Adipoq allele replaces exon 2 with neo. TK, thymidine kinase. B: Southern blot demonstrating disruption of the Adipoq locus. Targeted allele is identified by an ~4-kb decrease in an XbaI restriction fragment that is detected with a 525-bp probe located downstream of the targeting vector sequences. Absence of adiponectin in the serum of adiponectin-deficient (Adipoq−/−) mice demonstrated by ELISA (C) and immunoblotting (D). ND, not detectable; Adipo, full-length adiponectin; gAdipo, purified globular head domain of murine adiponectin.