FIG. 4.
HPLC profile of glycerophospholipid molecular species in the total membrane and DRM from Caco-2 cells. The glycerophospholipids isolated from total membranes or DRM of Caco-2 cells were treated with phospholipase C, and the diglycerides obtained were benzoylated as described in Materials and Methods. These derivatives were analyzed by HPLC using an RP-C18 column and a mixture of acetonitrile-isopropan-2-ol (75/25 [vol/vol]) and quantified through their absorption at 230 nm. Peak identification has been already described (38) and is reported on Table 2.