FIG. 1.
Upregulation of cytokine receptor expression. HeLa 229 cells were treated in triplicate increasing concentrations of IFN-γ, IL-1β, TNF-α, or complete medium alone for 24 h. The relative amount of cytokine receptor expression was assessed by incubating cells with antibodies to specific receptor components followed by incubation with FITC-conjugated or PE-conjugated secondary antibodies and analyzing by flow cytometry. (A) Effect of IL-1 β on IFNGR expression. (B) Effect of TNF on IFNGR expression. (C) Effect of IFN-γ on TNFR expression. (D) Effect of IL-1 β on TNFR expression. (E) Effect of TNF-α on IL-1R expression. (F) Effect of IFN-γ on IL-1R expression. The results from one of three similar experiments (MFI\m=+-SD) are presented.
