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. 1988 Jun;72(3):450–456.

Measurement and characterisation of circulating anti-endothelial cell IgG in connective tissue diseases.

J Rosenbaum 1, B E Pottinger 1, P Woo 1, C M Black 1, S Loizou 1, M A Byron 1, J D Pearson 1
PMCID: PMC1541580  PMID: 3168322

Abstract

We have detected circulating IgG antibodies that bind with high affinity to human umbilical vein endothelial cells in 74% of patients with systemic lupus erythematosus (SLE), 30% of those with scleroderma and 28% of those with rheumatoid arthritis. IgG binding was F(ab) mediated, and did not involve immune complexes. Anti-endothelial IgG were apparently unrelated to other circulating autoantibodies, including anti-cardiolipin or antiDNA IgG. Bound IgG from SLE or scleroderma patients was displaced by IgG from certain unrelated patients whereas others were ineffective. Anti-endothelial cell IgG from all sera tested were adsorbed by human dermal fibroblasts; erythrocytes and leucocytes each adsorbed a fraction of the activity. Purified IgG did not induce complement-mediated cytotoxicity. We conclude that a discrete group of IgG antibodies is common in connective tissue disease patients, reacts predominantly with endothelial cells and dermal fibroblasts, and may be important in the pathogenesis of vascular damage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alcocer-Varela J., Martinez-Cordero E., Alarcon-Segovia D. Spontaneous production of, and defective response to, interleukin-1 by peripheral blood mononuclear cells from patients with scleroderma. Clin Exp Immunol. 1985 Mar;59(3):666–672. [PMC free article] [PubMed] [Google Scholar]
  2. Alderuccio F., Barnett A. J., Campbell J. H., Pedersen J. S., Toh B. H. Scl-95/100: doublet of endothelial marker autoantigens in progressive systemic sclerosis. Clin Exp Immunol. 1986 Apr;64(1):94–100. [PMC free article] [PubMed] [Google Scholar]
  3. Black C. M., Duance V. C., Sims T. J., Light N. D. An investigation of the biochemical and histological changes in the collagen of the kidney and skeletal muscle in systemic sclerosis. Coll Relat Res. 1983 May;3(3):231–243. doi: 10.1016/s0174-173x(83)80006-5. [DOI] [PubMed] [Google Scholar]
  4. Cariou R., Tobelem G., Soria C., Caen J. Inhibition of protein C activation by endothelial cells in the presence of lupus anticoagulant. N Engl J Med. 1986 May 1;314(18):1193–1194. doi: 10.1056/NEJM198605013141817. [DOI] [PubMed] [Google Scholar]
  5. Cines D. B., Lyss A. P., Reeber M., Bina M., DeHoratius R. J. Presence of complement-fixing anti-endothelial cell antibodies in systemic lupus erythematosus. J Clin Invest. 1984 Mar;73(3):611–625. doi: 10.1172/JCI111251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen S., Johnson A. R., Hurd E. Cytotoxicity of sera from patients with scleroderma. Effects on human endothelial cells and fibroblasts in culture. Arthritis Rheum. 1983 Feb;26(2):170–178. doi: 10.1002/art.1780260208. [DOI] [PubMed] [Google Scholar]
  7. Cotran R. S., Gimbrone M. A., Jr, Bevilacqua M. P., Mendrick D. L., Pober J. S. Induction and detection of a human endothelial activation antigen in vivo. J Exp Med. 1986 Aug 1;164(2):661–666. doi: 10.1084/jem.164.2.661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Drenk F., Mensing H., Serbin A., Deicher H. Studies on endothelial cell cytotoxic activity in sera of patients with progressive systemic sclerosis, Raynaud syndrome, rheumatoid arthritis, and systemic lupus erythematosus. Rheumatol Int. 1985;5(6):259–263. doi: 10.1007/BF00541353. [DOI] [PubMed] [Google Scholar]
  9. Fauci A. S., Haynes B., Katz P. The spectrum of vasculitis: clinical, pathologic, immunologic and therapeutic considerations. Ann Intern Med. 1978 Nov;89(5 Pt 1):660–676. doi: 10.7326/0003-4819-89-5-660. [DOI] [PubMed] [Google Scholar]
  10. Freyssinet J. M., Wiesel M. L., Gauchy J., Boneu B., Cazenave J. P. An IgM lupus anticoagulant that neutralizes the enhancing effect of phospholipid on purified endothelial thrombomodulin activity--a mechanism for thrombosis. Thromb Haemost. 1986 Jun 30;55(3):309–313. [PubMed] [Google Scholar]
  11. Halliday G. M., Salaman M. R., Seifert M. H., Johnson K. J., Malcolm A. D. Evaluation of an ELISA system for determination of class-specific antibodies to native and denatured DNA in man. Ann Rheum Dis. 1985 Aug;44(8):507–513. doi: 10.1136/ard.44.8.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Harris E. N., Gharavi A. E., Tincani A., Chan J. K., Englert H., Mantelli P., Allegro F., Ballestrieri G., Hughes G. R. Affinity purified anti-cardiolipin and anti-DNA antibodies. J Clin Lab Immunol. 1985 Aug;17(4):155–162. [PubMed] [Google Scholar]
  13. Hashemi S., Smith C. D., Izaguirre C. A. Anti-endothelial cell antibodies: detection and characterization using a cellular enzyme-linked immunosorbent assay. J Lab Clin Med. 1987 Apr;109(4):434–440. [PubMed] [Google Scholar]
  14. Horneland M., Rekvig O. P., Jørgensen L., Hannestad K. Cultured human endothelial cells display an antigen that is recognized by certain human anti-chromatin autoantibodies. Clin Exp Immunol. 1983 Nov;54(2):373–377. [PMC free article] [PubMed] [Google Scholar]
  15. Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kahaleh M. B., Sherer G. K., LeRoy E. C. Endothelial injury in scleroderma. J Exp Med. 1979 Jun 1;149(6):1326–1335. doi: 10.1084/jem.149.6.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lafer E. M., Rauch J., Andrzejewski C., Jr, Mudd D., Furie B., Furie B., Schwartz R. S., Stollar B. D. Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids. J Exp Med. 1981 Apr 1;153(4):897–909. doi: 10.1084/jem.153.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Le Roux G., Wautier M. P., Guillevin L., Wautier J. L. IgG binding to endothelial cells in systemic lupus erythematosus. Thromb Haemost. 1986 Oct 21;56(2):144–146. [PubMed] [Google Scholar]
  19. Leung D. Y., Geha R. S., Newburger J. W., Burns J. C., Fiers W., Lapierre L. A., Pober J. S. Two monokines, interleukin 1 and tumor necrosis factor, render cultured vascular endothelial cells susceptible to lysis by antibodies circulating during Kawasaki syndrome. J Exp Med. 1986 Dec 1;164(6):1958–1972. doi: 10.1084/jem.164.6.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Loizou S., McCrea J. D., Rudge A. C., Reynolds R., Boyle C. C., Harris E. N. Measurement of anti-cardiolipin antibodies by an enzyme-linked immunosorbent assay (ELISA): standardization and quantitation of results. Clin Exp Immunol. 1985 Dec;62(3):738–745. [PMC free article] [PubMed] [Google Scholar]
  21. Meyer O., Haïm T., Dryll A., Lansaman J., Ryckewaert A. Vascular endothelial cell injury in progressive systemic sclerosis and other connective tissue diseases. Clin Exp Rheumatol. 1983 Jan-Mar;1(1):29–34. [PubMed] [Google Scholar]
  22. Pearson J. D., Slakey L. L., Gordon J. L. Stimulation of prostaglandin production through purinoceptors on cultured porcine endothelial cells. Biochem J. 1983 Jul 15;214(1):273–276. doi: 10.1042/bj2140273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Penning C. A., Cunningham J., French M. A., Harrison G., Rowell N. R., Hughes P. Antibody-dependent cellular cytotoxicity of human vascular endothelium in systemic sclerosis. Clin Exp Immunol. 1984 Sep;57(3):548–556. [PMC free article] [PubMed] [Google Scholar]
  24. Penning C. A., French M. A., Rowell N. R., Hughes P. Antibody-dependent cellular cytotoxicity of human vascular endothelium in systemic lupus erythematosus. J Clin Lab Immunol. 1985 Jul;17(3):125–130. [PubMed] [Google Scholar]
  25. Ramirez F., Williams R. C., Jr, Sibbitt W. L., Jr, Searles R. P. Immunoglobulin from systemic lupus erythematosus serum induces interferon release by normal mononuclear cells. Arthritis Rheum. 1986 Mar;29(3):326–336. doi: 10.1002/art.1780290304. [DOI] [PubMed] [Google Scholar]
  26. Summers G. D., Weiss J. B., Jayson M. I. Failure of sera from patients with scleroderma to exhibit cytotoxicity towards human umbilical vein endothelial cells. Rheumatol Int. 1984;5(1):9–13. doi: 10.1007/BF00541359. [DOI] [PubMed] [Google Scholar]
  27. Tan E. M., Rodnan G. P., Garcia I., Moroi Y., Fritzler M. J., Peebles C. Diversity of antinuclear antibodies in progressive systemic sclerosis. Anti-centromere antibody and its relationship to CREST syndrome. Arthritis Rheum. 1980 Jun;23(6):617–625. doi: 10.1002/art.1780230602. [DOI] [PubMed] [Google Scholar]
  28. Tannenbaum S. H., Finko R., Cines D. B. Antibody and immune complexes induce tissue factor production by human endothelial cells. J Immunol. 1986 Sep 1;137(5):1532–1537. [PubMed] [Google Scholar]
  29. Thornton S. C., Mueller S. N., Levine E. M. Human endothelial cells: use of heparin in cloning and long-term serial cultivation. Science. 1983 Nov 11;222(4624):623–625. doi: 10.1126/science.6635659. [DOI] [PubMed] [Google Scholar]

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