FIG. 6.
Inhibition of luciferase gene expression in EMC-Luc RNA-transfected L929 cells by SB203580. L929 cells were transfected with EMC-Luc RNA and then incubated for 30 min. (A) The cells were washed and incubated for the indicated times with (solid circles) or without (open circles) 40 μM SB203580, and luciferase gene expression was analyzed. Bars represent standard deviations. (B) Luciferase gene expression was analyzed at 24 h after treatment with the indicated concentrations of SB203580. Untransfected (trans−) and transfected cells without SB203580 treatment (trans+) were used as controls. The expression of luciferase generated from EMC-Luc RNA in each group was calculated as a percentage of the average expression of luciferase in trans+ cells. All experiments were performed in triplicate. Bars represent standard deviation. *, P < 0.01 as compared with transfected cells without SB203580. (C) Cells were labeled with [35S]methionine for 30 min after treatment with the indicated concentrations of SB203580 for 24 h, and proteins were analyzed by SDS-PAGE and subsequent autoradiography. Untransfected (trans−) and transfected cells without SB203580 treatment (trans+) were used as controls.