FIG. 4.
Reversibility of neutralization by MAb 2F5 and the T-20 peptide. (A) Comparison of IgG1b12, 2F5, and T-20 in standard (open circles) and preattachment (closed circles) neutralization assays. (B) The T-20 peptide and 2F5 MAb were compared in neutralization assays with modified wash steps. Symbols are as follows: open circles, standard assay; closed triangles, postattachment assay; closed circles, standard assay without a pre-DTT treatment wash step; open triangles, postattachment assay with an additional wash before the DTT incubation.