Figure 4.
Mutual information (Ii) and its statistical significance. (a) The Ii values for the RR in the prokaryotic two-component system. The abscissa and ordinate represent the sequence position and mutual information, respectively. The blue points are the observed values and the red points are the expected values. The error bars are drawn at three standard deviations from the mean of the expected value and correspond to P = 0.0013. (b) The distribution of Iis for the eukaryotic PKs in the AGC group. The abscissa and ordinate represent, respectively, the mutual information and the probability of observing a particular mutual information. The blue line is the observed result and the red line is the control from the method cited in ref. 34. The vertical line means that there is a probability of <0.001 of observing an Ii ≥ 1.0 in the control MSAs from the method cited in ref. 34. Note that “p” in this figure does not correspond to the P value described in the text, which has been determined by the linear transformation method from ref. 24. See Methods for details.